Amy Rosenzweig


Interdisciplinary Biological Sciences Program
Department of Molecular Biosciences

Office Phone: 847.467.5301
Fax: 847.467.6489
Rosenzweig Lab


Structural biology and bioinorganic chemistry; metal uptake, transport and storage; oxygen activation by metalloenzymes; biological methane oxidation; protein engineering; natural products biosynthesis; membrane protein crystallography

The Rosenzweig group is focused on understanding metalloprotein function on the molecular level. We use X-ray crystallographic, spectroscopic, biochemical, molecular biological, and genomic approaches to attack problems at the forefront of bioinorganic chemistry.
We are studying particulate methane monooxygenase (pMMO), an integral membrane metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria. The mechanism of pMMO and related enzymes remain one of the major unsolved problems in bioinorganic chemistry, and is the focus of intense attention as a route for biological gas-to-liquid conversion. Understanding how pMMO activates O2 for oxidation of methane and other hydrocarbons is the long term goal of this research program.  Current efforts are directed at high resolution structural characterization of the proposed dicopper active site, mechanistic studies, and developing protein catalysts based on pMMO. We are also investigating how methanotrophs acquire copper from the environment using a natural product called methanobactin. Current projects focus on elucidating both the methanobactin biosynthetic pathway and the mechanism of methanobactin transport. Finally, we are studying heavy metal transporters called P1B-type ATPases. Although these proteins are critical to metal homeostasis in all kingdoms of life, fundamental questions surrounding their structure, function, and coordination chemistry remain unresolved.

Selected Publications

Smith, A. T.; Barupala, D.; Stemmler, T. L.; Rosenzweig, A. C. Discovery and characterization of a novel metal binding domain involved in cadmium, cobalt, and zinc transport. Nat. Chem. Biol. 2015, 11, 678-684.

Sirajuddin, S.; Rosenzweig, A. C. Enzymatic oxidation of methane. Biochemistry 2015, 54, 2283-2294.

Culpepper, M. A.; Rosenzweig, A. C. Structure and protein-protein interactions of methanol dehydrogenase from Methylococcus capsulatus (Bath). Biochemistry 2014, 53, 6211-6219.

Culpepper, M. A.; Cutsail, G. E., III; Gunderson, W. A.; Hoffman, B. M.; Rosenzweig, A. C. Identification of the valence and coordination environment of the particulate methane monooxygenase copper centers by advanced EPR characterization. J. Am. Chem. Soc. 2014, 136, 11767-11775.

Sirajuddin, S.; Barupala, D.; Helling, S.; Marcus, K.; Stemmler, T. L.; Rosenzweig, A. C. Effects of zinc on particulate methane monooxygenase activity and structure. J. Biol. Chem. 2014, 289, 21782-21794.

Smith, A. T.; Smith, K. P.; Rosenzweig, A. C. Diversity of the metal-transporting P1B-type ATPases. J. Biol. Inorg. Chem. 2014, 6, 947-960.

Zielazinski, E. L.; González-Guerrero, M.; Subramanian, P.; Stemmler, T. L.; Argüello, J. M.; Rosenzweig, A. C. Sinorhizobium meliloti Nia is a P1B-5-ATPase expressed in the nodule during plant symbiosis and is involved in Ni and Fe transport. Metallomics 2013, 5, 1614-1623.

Kenney, G. E.; Rosenzweig, A. C. Genome mining for methanobactins. BMC Biol. 2013, 11, 17.

Culpepper, M. A.; Cutsail, G. E., III; Hoffman, B. M.; Rosenzweig, A. C. Evidence for oxygen binding at the active site of particulate methane monooxygenase. J. Am. Chem. Soc. 2012, 134, 7640-7643.

Kenney, G. E.; Rosenzweig, A. C. Chemistry and biology of the copper chelator methanobactin. ACS Chem. Biol. 2012, 7, 260-268.

Smith, S. M.; Rawat, S.; Telser, J.; Hoffman, B. M.; Stemmler, T. L.; Rosenzweig, A. C. Crystal structure and characterization of particulate methane monooxygenase from Methylocystis species strain M. Biochemistry 2011, 50, 10231-10240.

Balasubramanian, R.; Kenney, G. E.; Rosenzweig, A. C. Dual pathways for copper uptake by methanotrophic bacteria. J. Biol. Chem. 2011, 286, 37313-37319.

Boal, A. K.; Cotruvo, J. A., Jr.; Stubbe, J.; Rosenzweig, A. C. Structural basis for activation of class Ib ribonucleotide reductase. Science 2010, 329, 1526-1530.

Balasubramanian, R.; Smith, S. M.; Rawat, S.; Yatsunyk, L. A.; Stemmler, T. L.; Rosenzweig, A. C. Oxidation of methane by a biological dicopper center.  Nature 2010, 465, 115-119.



View all publications by publications by Amy C. Rosenzweig listed in the National Library of Medicine (PubMed).

Recent Photos

October 27, 2015